by Ulrich Hübscher (University of Zurich, Switzerland), Silvio Spadari (Institute of Molecular Genetics IGM-CNR, Italy), Giuseppe Villani (CNRS-Université Paul Sabatier, Toulouse, France), & Giovanni Maga (Institute of Molecular Genetics IGM-CNR, Italy)

Table of Contents (116k)
Preface (157k)
Chapter 1: History and Discovery of DNA Polymerases (1,408k)

Maintenance of the information embedded in the genomic DNA sequence is essential for life. DNA polymerases play pivotal roles in the complex processes that maintain genetic integrity. Besides their tasks in vivo, DNA polymerases are the workhorses in numerous biotechnology applications such as the polymerase chain reaction (PCR), cDNA cloning, genome sequencing, nucleic acids-based diagnostics and in techniques to analyze ancient and otherwise damaged DNA. Moreover, some diseases are related to DNA polymerase defects, and chemotherapy through inhibition of DNA polymerases is used to fight HIV, Herpes and Hepatitis B and C infections. We have recently witnessed the discovery of an abundance of novel DNA polymerases in viruses, bacteria, archaea and eukaryotes with specialized properties whose physiological functions are only beginning to be understood. This book summarizes the current knowledge of these fascinating enzymes. It is intended for a wide audience from basic scientists, to diagnostic laboratories and to clinicians who seek a better understanding of these fascinating enzymes.

• History and Discovery of DNA Polymerases
• DNA Polymerases in the Three Kingdoms of Life: Bacteria, Archaea and Eukaryotes
• Structural and Functional Aspects of the Prokaryotic and Archaea DNA Polymerase Families
• Structural and Functional Aspects of the Eukaryotic DNA Polymerase Families
• Global Functions of DNA Polymerases
• Viral DNA Polymerases
• Synthetic Evolution of DNA Polymerases with Novel Properties
• DNA Polymerases and Diseases
• DNA Polymerases and Chemotherapy