edited by Jason S Kim (Naval Research Laboratory, USA) & Frances S Ligler (Naval Research Laboratory, USA)

This book will describe the continuing development of inexpensive, portable flow cytometers through incorporation of microfluidic technologies and small optical components. The underlying microfluidic theories essential for microflow cytometry will be discussed in detail, as well as advances that are representative of the current state-of-the-art. Design and fabrication strategies for these innovative component technologies will be subsequently presented by numerous research groups leading the field. Integration of the components into functional prototype devices for analysis and manipulation of particles and cells will be reviewed. Multiple currently available commercial systems will be examined to highlight both strengths and areas for improvement.

• The History of Flow Cytometry and Sorting (H Shapiro)
• Analysis of Single Cells Using Lab-on-a-Chip Systems (T Preckel)
• Luminex Personal Flow Cytometers (W Roth)
• The Accuri C6 Flow Cytometer (C Rich & G Howes)
• Capillary Flow Cytometry (D King et al.)
• Sheathless Particle Focusing (S Choi et al.)
• Two-Dimensional Particle Focusing (J Shin & M Ladisch)
• Three-Dimensional Particle Focusing (P Howell)
• Fluidic Control: Pumps and Valves (S Zheng et al.)
• Integrated Optics (Y Hosseini & K Kaler)
• Polymer Photonics (D Leuenberger & M Ramuz)
• Electrical Detection (M Di Berardino)
• Electronics (J Erickson et al.)
• Optical Micro-FACS (K Patel & T Perroud)
• Raman-based Label-free Cell Analysis and Sorting (J Chan)
• Autonomous Pathogen Detection System (J Dzenitis & A Makarewicz)
• Laser-based Fabrication with Integrated Optical Waveguides (M Dugan et al.)
• Systems Integration (J Kim et al.)